l johnsonii strain  (ATCC)

Journal: Frontiers in Microbiology

Article Title: Remodeling of the gut microbiome by Lactobacillus johnsonii alleviates the development of acute myocardial infarction

doi: 10.3389/fmicb.2023.1140498

Figure Lengend Snippet: Lactobacillus johnsonii EU03 protects cardiac function after AMI. (A) The schematic diagram for L. johnsonii administration pipeline in rats after AMI. (B) Representative echocardiograph of rats left ventricle. (C) EF and (D) FS calculated using dimensional measurements of rats left ventricle. (E) Representative heart pictures, H&E staining [scale bar equals 3,000 μm (top) and 125 μm (bottom)], Wheat germ aggregates (WGA) staining (scale bar equals 50 μm), and Sirius Red staining (scale bar equals 100 μm). Serum levels of (F) SOD, (G) MDA, (H) LDH, (I) CK-MB, (J) cTnT, (K) TNF-α, and (L) LPS. # p < 0.05, ## p < 0.01 versus Sham and * p < 0.05, * p < 0.01 versus AMI.

Article Snippet: L. johnsonii strain EU03 is deposited in the China Pharmaceutical Culture Collection (CPCC) under accession number CPCC101288 and China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC20845 ( ).

Techniques: Staining

Journal: Frontiers in Microbiology

Article Title: Remodeling of the gut microbiome by Lactobacillus johnsonii alleviates the development of acute myocardial infarction

doi: 10.3389/fmicb.2023.1140498

Figure Lengend Snippet: L. johnsonii EU03 improves intestinal barrier and affects the composition of gut microbiome after AMI. (A) Histopathological examination of colon tissue by H&E staining [scale bar equals 1,250 μm (top) and 125 μm (bottom)], Alcian blue (AB) staining (scale bar equals 100 μm), and representative immunofluorescence photomicrographs for tight junction proteins zonula occludentes (ZO)-1 (red) and occludin (green) in the colonic epithelium (scale bar equals 125 μm). (B) The ZO-1 and (C) occludin intensity were quantified by calculating integrated optical density (IOD). (D) Beta diversity of bray curtis-based PCoA of different groups for 7, 14, and 28 days administration, and different time plots together. Significant p values of Anosim between groups emphasize the differences in microbial community structure. (E) Alpha diversity of Shannon index in each group. (F) F/B ratio of different groups. (G) Kruskal-Wallis H test bar graph for specific microbial composition at genus and species levels. (H) Species correlation network at the species level. The correlation coefficient of Spearman between species was calculated to reflect the correlation between species. By default, the Figure shows the species with p < 0.05; the size of nodes indicates corresponding bacteria abundance, red line indicates positive correlation while the green line indicates negative correlation; a greater number of lines indicates closer relationship between species. (I) LEfSe at different levels of each group. The length of the histogram represents the abundance of the difference species (i.e., LDA score). “g for genus” and “s for_species.” # p < 0.05, ## p < 0.01, ### p < 0.001versus Sham and * p < 0.05, ** p < 0.01, *** p < 0.001 versus AMI.

Article Snippet: L. johnsonii strain EU03 is deposited in the China Pharmaceutical Culture Collection (CPCC) under accession number CPCC101288 and China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC20845 ( ).

Techniques: Staining, Immunofluorescence, Bacteria

Journal: Frontiers in Microbiology

Article Title: Remodeling of the gut microbiome by Lactobacillus johnsonii alleviates the development of acute myocardial infarction

doi: 10.3389/fmicb.2023.1140498

Figure Lengend Snippet: Antibiotics (ABX) induced dysbacteriosis interferes the beneficial effects on cardiac function of L. johnsonii . (A) The schematic diagram for L. johnsonii and ABX administration pipeline in AMI rats. (B) Rats were treated with ABX for 7 days prior to LAD surgery. The sham group and the AMI group were treated daily with control saline, Lactobacillus alone or simultaneously with ABX for 28 days by oral gavage. (C) Representative echocardiograph of rat’s left ventricle. (D) EF, (E) FS, (F) LVID; d and (G) LVID; s calculated using dimensional measurements of rats left ventricle. (H) Representative heart pictures, H&E staining [scale bar equals 3,000 μm (top) and 75 μm (bottom)], WGA and Masson staining of heart tissue (scale bar equals 50 μm and 650 μm). # p < 0.05, ## p < 0.01, ### p < 0.001 versus Sham, * p < 0.05, ** p < 0.01 versus AMI, & p < 0.05 versus LJ and ^ p < 0.05, ^^ p < 0.01 versus AMI, LJ, LR, correspondingly.

Article Snippet: L. johnsonii strain EU03 is deposited in the China Pharmaceutical Culture Collection (CPCC) under accession number CPCC101288 and China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC20845 ( ).

Techniques: Control, Saline, Staining

Journal: BMC Microbiology

Article Title: Effective prophylaxis against rotavirus diarrhea using a combination of Lactobacillus rhamnosus GG and antibodies

doi: 10.1186/1471-2180-7-86

Figure Lengend Snippet: Outcome measures of diarrhea among mice receiving different probiotic bacteria and challenged with RRV

Article Snippet: Four different Lactic acid bacteria were obtained from Nestec, Nestlé, Lausanne, L. paracasei strain NCC 2461 (ST11), L. rhamnosus strain GG (ATCC 53103), L. johnsonii strain NCC 533 (La-1) [ ] , L. rhamnosus strain NCC 596 and Streptococcus thermophilus strain NCC 2496.

Techniques: Infection

Journal: Frontiers in Pharmacology

Article Title: Gut microbiome and human health: Exploring how the probiotic genus Lactobacillus modulate immune responses

doi: 10.3389/fphar.2022.1042189

Figure Lengend Snippet: Animal and clinical studies of different Lactobacillus strains in alleviating inflammatory diseases pertaining to different organs.

Article Snippet: The proposed mechanism of action includes: 1) Bile salts degradation through the action of microbial bile salt hydrolase (BSH), making them relatively less soluble and thus reducing their reabsorption by the intestinal epithelial layer and increasing their excretion in feces as observed in L. gasseri LBM220 ( ) and L. mucosae SRV5 and SRV10 ( ) 2) Microbial conversion of cholesterol to relatively soluble form coprostanol which can readily assimilate and excreted out via feces 3) Incorporation of cholesterol by the microbial membrane as observed in strain L. acidophilus ATCC 43121 4) production of short chain fatty acids (SCFA).

Techniques: Expressing, Blocking Assay